wnt signaling pathway inhibitor xav939  (MedChemExpress)

Journal: Translational Andrology and Urology

Article Title: Comprehensive bioinformatics and experimental analysis of PPOX reveals its carcinogenic effect in clear cell renal cell carcinoma

doi: 10.21037/tau-2026-1-0024

Figure Lengend Snippet: PPOX knockdown inhibits the migratory and invasive capacities of ccRCC cells and downregulates Wnt/β-catenin signaling pathway activity. (A) Wound healing assay confirmed that PPOX knockdown inhibited cell migration (magnification, 40×). (B) Transwell assay confirmed that PPOX knockdown inhibited cell invasion (stained with crystal violet; magnification, 100×). (C) PPOX knockdown reduced Wnt/β-catenin pathway activity. All experiments were performed with at least three independent replicates. Statistical comparisons between two groups were analyzed by unpaired Student’s t-test using GraphPad Prism 8. Scale bar: 100 μm. NC, negative control; PPOX, protoporphyrinogen oxidase.

Article Snippet: The canonical Wnt/β-catenin pathway was activated using CHIR-99021 (MedChemExpress, Cat# HY-10182), a selective glycogen synthase kinase-3β (GSK-3β) inhibitor.

Techniques: Knockdown, Activity Assay, Wound Healing Assay, Migration, Transwell Assay, Staining, Negative Control

Journal: Translational Andrology and Urology

Article Title: Comprehensive bioinformatics and experimental analysis of PPOX reveals its carcinogenic effect in clear cell renal cell carcinoma

doi: 10.21037/tau-2026-1-0024

Figure Lengend Snippet: PPOX overexpression enhances the proliferative, migratory, and invasive capacities of ccRCC cells and upregulates Wnt/β-catenin pathway activity. (A) PPOX overexpression efficiency in A498 and 769-P cells. (B-D) CCK-8, colony formation, and EdU assays (EdU and DAPI staining; magnification, 100×) confirmed that cell proliferation was elevated after PPOX overexpression. (E,F) Wound healing (40×) and Transwell assays (stained with crystal violet; magnification, 100×) confirmed that PPOX overexpression enhances cell migration and invasion. (G) PPOX overexpression enhanced Wnt/β-catenin pathway activity. All experiments were performed with at least three independent replicates. Statistical comparisons between two groups were analyzed by unpaired Student’s t-test using GraphPad Prism 8. Scale bar: 100 μm. NC, negative control; OD, optical density; OE, overexpression; PPOX, protoporphyrinogen oxidase.

Article Snippet: The canonical Wnt/β-catenin pathway was activated using CHIR-99021 (MedChemExpress, Cat# HY-10182), a selective glycogen synthase kinase-3β (GSK-3β) inhibitor.

Techniques: Over Expression, Activity Assay, CCK-8 Assay, Staining, Migration, Negative Control

Journal: Translational Andrology and Urology

Article Title: Comprehensive bioinformatics and experimental analysis of PPOX reveals its carcinogenic effect in clear cell renal cell carcinoma

doi: 10.21037/tau-2026-1-0024

Figure Lengend Snippet: PPOX regulates malignant progression of ccRCC through the Wnt/β-catenin pathway. (A) TOP/FOP Flash reporter activity ratios in A498 and 769-P cells following PPOX knockdown or overexpression. (B) Protein expression levels of β-catenin, c-Myc and CCND1 in A498 and 769-P cells after PPOX knockdown and CHIR-99021 treatment. (C) Cell proliferation assessed by EdU assays (magnification, 100×) under PPOX knockdown and CHIR-99021 treatment. (D) Cell migration evaluated by wound healing assays (magnification, 40×). (E) Cell invasion analyzed by Transwell assays (stained with crystal violet; magnification, 100×). All experiments were performed with at least three independent replicates. Two-group comparisons were analyzed using the unpaired t-test, whilst multiple groups (>2) were compared using one-way ANOVA, followed by Tukey’s HSD test for post hoc pairwise comparisons where applicable. Scale bar: 100 μm. ANOVA, analysis of variance; ccRCC, clear cell renal cell carcinoma; HSD, Honestly Significant Difference; NC, negative control; PPOX, protoporphyrinogen oxidase.

Article Snippet: The canonical Wnt/β-catenin pathway was activated using CHIR-99021 (MedChemExpress, Cat# HY-10182), a selective glycogen synthase kinase-3β (GSK-3β) inhibitor.

Techniques: Activity Assay, Knockdown, Over Expression, Expressing, Migration, Staining, Negative Control

Journal: Frontiers in Oncology

Article Title: Effects of SOX10 on the proliferative, invasive, migratory, and epithelial–mesenchymal transition abilities of triple-negative breast cancer cells

doi: 10.3389/fonc.2026.1769833

Figure Lengend Snippet: Effect of SOX10 knockdown and overexpression on classical Wnt/β-catenin pathway-related protein expression in TNBC cell lines. Protein expression of C-myc, CyclinD1, WNT1, phospho-GSK-3β (Ser9), and nuclear β-catenin was detected by Western blot analysis, with band gray values normalized to GAPDH (total protein) or Lamin B1 (nuclear protein, for β-catenin). (A) Protein expression and quantification in MDA-MB-453 cells. (B) Protein expression and quantification in HCC1937 cells. All experiments were independently repeated three times (n = 3 independent biological replicates), and representative results are shown. Statistical analysis was performed using unpaired two-tailed Student’s t-test for two-group comparisons. siSOX10 vs. siNC, ** P < 0.01; SOX10-OEvs. NC, ## P < 0.01. && indicates P<0.01; $$ indicates P<0.01 for statistical comparisons.

Article Snippet: The Wnt/β-catenin pathway was activated using CHIR-99021 (a selective GSK-3β inhibitor; MedChemExpress, Monmouth Junction, New Jersey, United States, Cat. No. HY-10182) at a concentration of 5 μM , and inhibited using XAV939 (a tankyrase 1/2 inhibitor; MedChemExpress, Cat. No. HY-15147) at a concentration of 10 μM ( ).

Techniques: Knockdown, Over Expression, Expressing, Western Blot, Two Tailed Test

Journal: Frontiers in Oncology

Article Title: Effects of SOX10 on the proliferative, invasive, migratory, and epithelial–mesenchymal transition abilities of triple-negative breast cancer cells

doi: 10.3389/fonc.2026.1769833

Figure Lengend Snippet: SOX10 promotes EMT in MDA-MB-453 cells via activating the classical Wnt/β-catenin signaling pathway. (A) Relative protein expression of Wnt/β-catenin pathway-related factors (WNT1, β-catenin, C-myc, and CyclinD1) in siSOX10 MDA-MB-453 cells treated with a Wnt activator, and SOX10-overexpressing MDA-MB-453 cells treated with a Wnt inhibitor. (B) Relative protein expression of EMT-related markers (E-cadherin, N-cadherin, and Vimentin) in the aforementioned treatment groups. All protein levels were detected by Western blot analysis, with band gray values normalized to GAPDH. All experiments were independently repeated three times (n = 3 independent biological replicates), and representative results are shown. Statistical analysis was performed using one-way ANOVA with Tukey’s post-hoc test for multiple comparisons among groups. Compared with MDA-MB-453+siNC, * P < 0.05, ** P < 0.01; compared with MDA-MB-453+siSOX10, # P < 0.05, ## P < 0.01; compared with MDA-MB-453+NC.

Article Snippet: The Wnt/β-catenin pathway was activated using CHIR-99021 (a selective GSK-3β inhibitor; MedChemExpress, Monmouth Junction, New Jersey, United States, Cat. No. HY-10182) at a concentration of 5 μM , and inhibited using XAV939 (a tankyrase 1/2 inhibitor; MedChemExpress, Cat. No. HY-15147) at a concentration of 10 μM ( ).

Techniques: Expressing, Western Blot

Journal: Frontiers in Oncology

Article Title: Effects of SOX10 on the proliferative, invasive, migratory, and epithelial–mesenchymal transition abilities of triple-negative breast cancer cells

doi: 10.3389/fonc.2026.1769833

Figure Lengend Snippet: SOX10 promotes EMT in HCC1937 cells via activating the classical Wnt/β-catenin signaling pathway. (A) Relative protein expression of Wnt/β-catenin pathway-related factors (WNT1, β-catenin, C-myc, and CyclinD1) in siSOX10 HCC1937 cells treated with a Wnt activator, and SOX10-overexpressing HCC1937 cells treated with a Wnt inhibitor. (B) Relative protein expression of EMT-related markers (E-cadherin, N-cadherin, and Vimentin) in the aforementioned treatment groups. All protein levels were detected by Western blot analysis, with band gray values normalized to GAPDH. All experiments were independently repeated three times (n = 3 independent biological replicates), and representative results are shown. Statistical analysis was performed using one-way ANOVA with Tukey’s post-hoc test for multiple comparisons among groups. Compared with HCC1937+siNC, ** P < 0.01; compared with HCC1937+siSOX10, ## P < 0.01; compared with HCC1937+NC.

Article Snippet: The Wnt/β-catenin pathway was activated using CHIR-99021 (a selective GSK-3β inhibitor; MedChemExpress, Monmouth Junction, New Jersey, United States, Cat. No. HY-10182) at a concentration of 5 μM , and inhibited using XAV939 (a tankyrase 1/2 inhibitor; MedChemExpress, Cat. No. HY-15147) at a concentration of 10 μM ( ).

Techniques: Expressing, Western Blot